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Biomarkers for lipid oxidation. oxidative stress
Anti 4-HNE monoclonal antobody (HNE-J2)
Suitable for immunohistocheistry and western blotting. For research use only.
About 4-hydroxy-2-nonenal (4-HNE)

Structure of 4-hydroxy-2-nonenal
Reactive oxygen species (ROS) are involved in lipid peroxidation inside the living bodies, and membrane lipids are one of the major targets of ROS. During the peroxidation process, a variery of aldehydes are formed. 4-hydroxy-2-nonenal (4-HNE) is an alpha, beta unsatulated aldehyde that can be formed by peroxidation of omega-6 unsatulated fatty acids such as linoleic acid and arachidonic acid. Especially, 4-HNE formed inside living body is reported to originate from phospholipid-bound arachidonic acid. 4-HNE may be one of the most reliable biomarker of lipid peroxidation.
High-power view of colorectal carcinoma cells. Adenocarcinoma (arrows) and nontumorous epithelial cells (star x240). (S. Kondo et al. Free Radical Biology & Medicine 27 pp401-410, 1999)
Specifications
Clone #: HNEJ-2
Immunogen: 4-HNE-modified Keyhole Limpet Hemocyanin
Subclass: Mouse IgG1(kappa)
Prepared as ascite, and ammnonium sulphate purified.
Form: Lyophilized Powder (containing 20 µg or 100 µg of IgG)
Specificity: This antibody show almost negligible reactivity with proteins that were treated with other aldehydes, such as 2-nonenal, 2-hexenal, 1-hexanal, 4-hydroxy-2-hexenal,formaldehyde, or glutaraldehyde.
Applications: Immunohistochemistry and western blotting.
Storage: Less than -20°C for 5 years. When transport, stable at least 7 days at room temperature.
References
Immunohistochemistry:
T.Tanaka, Y.Nishiyama, K.Okada, K.Hirota, M.Matsui, J.Yodoi, H.Hiai, and S.Toyokuni:
Induction and nuclear translocation of thioredoxin by oxidative damage in the mouse kidney: independence of tubular necrosis and sulfhydryl depletion. Lab. Invest. 77(2), p145-155 (1997)
Western blotting:
S.Toyokuni, N.Miyake, H.Hiai, M.Hagiwara, S.Kawakishi, T.Osawa and K.Uchida:
The monclonal antibody specific for the 4-hydroxy-2-nonenal histidine adduct.
FEBS Lett. 359, p189-191 (1995)
Product name Code Content
Anti 4-HNE monoclobal antibody MHN-020P 20 µg of IgG/ vial
MHN-100P 100 µg of IgG/ vial

Made in Japan

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Instruction manual (PDF) Immunohistochemical procedure Technical information
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